Gene expression profiling of murine embryonic and epiblast stem cells under IQ1 treatment and Ctnnb1 K49 acetylation conditions

Naïve pluripotency represents the ground state of mammalian development, but the molecular mechanisms controlling its establishment remain incompletely defined. To clarify the role of β-catenin (Ctnnb1) acetylation, we performed microarray-based transcriptome profiling of murine embryonic stem cells (ESCs, naïve) and epiblast stem cells (EpiSCs, primed) under different culture conditions. Particular focus was placed on the acetylation of Ctnnb1 at lysine 49 (K49) and its modulation by IQ1, a small molecule that inhibits Ep300 function and enhances K49 acetylation. This dataset enables comparison of gene expression signatures between naïve and primed states and provides insights into the induction of naïve-like characteristics in EpiSCs following IQ1 treatment. Naïve pluripotency is the developmental ground state. To define transcriptional programs associated with β-catenin (Ctnnb1) lysine-49 acetylation, we performed microarray profiling of mouse embryonic stem cells (ESCs; naïve), epiblast stem cells (EpiSCs; primed), and EpiSCs exposed to IQ1 to increase K49 acetylation. The dataset enables direct comparison of naïve versus primed states and assessment of IQ1-driven conversion toward a naïve-like transcriptome.