Gene expression in Achilles tendon cells of SD rats after 1 h of running followed by 12 h of rest

Achilles tendinopathy is often thought to be a consequence of overuse of the Cells within the Achilles tendon of healthy rats undergo a series of changes following physiologic levels of mechanical stimulation after running, and we further explored the transcriptome changes in Achilles tendon cells during post-exercise recovery. Our current experiment reveals RNA-seq analysis of the transcriptome of the rat Achilles tendon after 12 hours of rest following running. Overall design: Rats in the running group (12 h p.r. group) sacrificed 12 h after 1 h of treadmill running. Control rats rested in cages and were sacrificed at the same time as the running group. Fresh Achilles tendon tissues from the right legs of the running and naïve control rats were immediately dissected and placed in RNALater™ to prevent RNA degradation. The total RNA was extracted after the tissues were mechanically pulverized in TRIzol. After verification of the RNA integrity by Qsep100 (RNA integrity number > 7.0), polyA mRNAs was purified from total RNA using mRNA capture beads. Then the mRNA was reverse-transcribed to create a cDNA library. VAHTS Stranded mRNA-seq Library Prep Kit for Illumina V2 was used for library preparation. RNA sequencing was performed using the Illumina NovaSeq 6000 sequencer. We then performed gene expression profiling analysis using data obtained from RNA-seq of 2 different tissues at two time points. Comparative gene expression profiling analysis of RNA-sea data for 0 h p.r. group and NC