Next Generation Sequencing (NGS) of brain BRAFV600E/pAkt/Trp53KO- and control-derived tissue

Purpose: Usage of next generation sequencing (NGS) to quantify the expression levels of the overall transcriptome from brain BRAFV600E/pAkt/Trp53KO tumors and and compare it with the contralateral brain control derived from mice. Methods: Tumor tissues and adjacent normal tissues from mouse brains were microdissected, frozen in liquid nitrogen and stored at -80 °C before RNA extraction. RNA was isolated using the RNeasy Micro Kit (Qiagen) following the manufacturer´s instructions and evaluated using a NanoDrop1000 spectrophotometer and a Qubit RNA HS Assay Kit on a Qubit 2.0 Fluorometer (Thermo Fisher Scientific). Conclusion: Comparison between the transcriptome of tumor and control brain tissue rise the presence of differentially expressed genes related to immune system, inflamation, proliferation, invasion, synaptic processes, etc. Tumor and control mRNA profiles of BRAFV600E/pAkt/Trp53KO and control brain mouse tissue