Zinc content of purified recombinant Har and C97A with and without metal chelator treatment.
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Protein solutions were treated with the strong metal chelator Chelex-100 at pH 5.0 or EDTA with or without 8 M urea at pH 8.0. After removal of the metal chelators and urea, the zinc ions in the protein solutions were determined by ICP-MS, with lysozyme as a negative control protein. anot detected. Zinc content of purified recombinant Har and C97A with and without metal chelator treatment.
创建时间：
2014-11-06
