LC-HRMS study of Arabidopsis root metabolome in wild-type Col-0 and npr1 mutant upon in vitro inoculation with Streptomyces sp. AgN23.

This archive compiles datasets related to studies of Streptomyces sp. AgN23 interaction with Arabidopsis thaliana. Ultra-high-performance liquid chromatography-high-resolution MS (UHPLC-HRMS) analyses were performed on a Q Exactive Plus quadrupole (Orbitrap) mass spectrometer, equipped with a heated electrospray probe (HESI II) coupled to a U-HPLC Ultimate 3000 RSLC system (Thermo Fisher Scientific, Hemel Hempstead, United Kigdom). For each biological sample, the RAW file obtained in ESI+ and ESI- mode were retrieved from the Xcalibur version 4.4 software and are deposited in separate sub-folders termed "RawPos" and "RawNeg". Each folder contains all the data relative to the cohort comprising "Blank" samples (n=13), Quality Check samples (pool of all samples from the cohort) "QC" (n=8), Control Col-0 plant samples "Col0CTRL" (n=6), Col-0 plant inoculated with AgN23 samples "Col0AgN23WT" (n=6), Control npr1 plant samples"NPR1CTRL" (n=6), npr1 plant inoculated with AgN23 samples "NPR1AgN23WT" (n=6). The files belonging to Negative mode bare the "neg" suffix and those belonging to Positive mode "pos" suffix, i.e. "Col0AgN23WT1_neg" and Col0AgN23WT1_pos". The details regarding samples preparation, analytic parameters and mass spectrometry, statistical treatment and visualization of the data will be made available in the publication relating to this archive.