Gene expression in Atlantic salmon cell culture in response to infectious salmon anaemia virus (ISAV) and polyinosinic-polycytidylic acid - poly(I:C)

ISAV is one of the most dangerous pathogens causing high mortality of farmed Atlantic salmon. In this study, transcriptome responses to the virus were examined in vitro in Atlantic salmon head kidney cells culture (ASK). Poly(I:C), synthetic double-stranded RNA stimulating antiviral responses was used as a positive control. Viral infection was performed at 15° C in 75cm2 flasks and cells were harvested after 1 and 5 days. Cells cultivated with added poly(I:C) were sampled at 5 days. Analyses were performed in two replicates.