Remodeling of Zn2+ Homeostasis Upon Differentiation of Mammary Epithelial Cells

Using mouse mammary epithelial cells as a model system, we investigated the remodeling of zinc homeostasis during differentiation induced by treatment with the lactogenic hormones cortisol and prolactin. RNA-Seq at different stages of differentiation revealed changes in global gene expression, including genes encoding zinc-dependent proteins and regulators of zinc homeostasis. Increases in mRNA levels of three zinc homeostasis genes,. 3 replicates of each of 3 sample groups (R day1, D day1, D day6). R day1 was used as the reference condition for all comparisons.