Source data: Native American ataxia medicines rescue ataxia-linked mutant potassium channel activity via binding to the voltage sensing domain

There are currently no drugs known to rescue the function of Kv1.1 voltage-gated potassium channels carrying loss-of-function sequence variants underlying the inherited movement disorder, Episodic Ataxia 1 (EA1). The Kwakwaka'wakw First Nations of the Pacific Northwest Coast used Fucus gardneri (bladderwrack kelp), Physocarpus capitatus (Pacific ninebark) and Urtica dioica (common nettle) to treat locomotor ataxia. Here, extracts of these plants enhanced wild-type Kv1.1 current, especially at subthreshold potentials. Screening of their constituents revealed that gallic acid and tannic acid similarly augmented wild-type Kv1.1 current, with submicromolar potency. Crucially, the extracts and their constituents also enhanced activity of Kv1.1 channels containing EA1-linked sequence variants. Molecular dynamics simulations revealed that gallic acid augments Kv1.1 activity via a previously unreported small-molecule binding site in the extracellular S1-S2 linker. Thus, traditional Native Ameri..., , , # Source data: Native American ataxia medicines rescue ataxia-linked mutant potassium channel activity via binding to the voltage sensing domain

The Excel files and molecular dynamic simulation files used for Figures 1 - 10 have been included as part of this repository.

### Description of Data

Excel files are organised into:

Figure>channel>drug/extract>analysis(e.g. Gmax, IV). Each Excel file is divided into individual tabs for control and concentration of compound/extract.

For molecular dynamic simulations, each file includes:

system_name.psf : topology information and forcefield atom types
system_name_initial.pdb: initial configuration coordinates
system_name_final.pdb: final configuration coordinates

where system_name is one of the following:

Simulation System system_name
Kv1.1 VSD kv11_popc_waters_ions
Kv1.1 VSD with gallic acid kv11_gala_popc_waters_ions
Kv1.1-3M kv11_3M_popc_waters_ions
Kv1.1-3M with gallic acid kv11_3M_2_gala_popc_waters_ions