Classification of the selected anti-Crf2 scFv gene fragments with human gene segments according to VBASE2.

Abbreviations: HV: V (variable) gene segments of the heavy chain; D: D (diversity) gene segment; HJ: J (joining) gene segment of the heavy chain; LV: V gene segment of the light chain; LJ: J gene segment of the light chain; HAL4/7: human, naive antibody gene library; immune library: immune library derived from Crf2 immunized macaque. The germinality index describes the similarity of the anti-Crf2 scFvs to the most similiar human germline genes identified by VBASE2 (www.vbase2.org) indicated by percental identity of the VH or VL framework region.