Differentially expressed noncoding RNAs as potential biomarkers for DIPG diagnosis and prognosis

The most fatal primary brainstem tumors in pediatric patients are diffuse intrinsic pontine gliomas (DIPGs). The identification of genetic alterations, such as the H3K27M mutation, allows a better molecular classification of these tumors. Therefore, the identification of new molecular features, mediating their formation and progression, as non-coding RNAs (ncRNAs), would be of great importance for the development of effective treatments. DIPGs transcriptome was analyzed with the HTA2.0 array and compared it with non-brain stem pediatric astrocytoma expression profiles obtained from the GEO database (GSE72269). Expression mRNA changes were validated by qPCR and physical interactions between lncRNAs and snoRNAs were bioinformatically predicted.