Mus musculus domesticus strain:Brn-3b KO mutant | isolate:aorta RNA | breed:C57BL6 Transcriptome or Gene expression. Mus musculus domesticus strain:Brn-3b KO mutant | isolate:aorta RNA | breed:C57BL6

The aim of this study was to identify target genes regulated by the POU4F2 also called Brn 3b transcription factor also called Brn 3b in controlling vascular integrity and function.This was achieved using RNA sequencing to analyse for genes that were differentially regulated in the aorta of Brn 3b KO mouse mutants in which the Brn-3b gene was constitutively deleted when compared with aged-matched, wild-type littermate controls.For these studies, highly purified mRNA extracted from 3 Brn 3b KO aortas and WT controls were used for RNA-dependent cDNA synthesis and first strand library preparation, prior to sequencing on the Illumina NextSeq 500 instrument using paired end run with general yields of approximately 15 million reads per sample.De-multiplexed data was converted to Fastq files on Illumina bcl2fastq Conversion Software v2.19, aligned with the reference genome using STAR v2.5b deduplicated using Picard Tools v2.7.1 and transcript reads were counted by FeatureCounts v1.4.6p5.Ensembl ID for each gene was determined using gProfiler and this data was then used for normalisation, modelling and differential expression analysis using the iDEP.93 software platform, which connects multiple R Bioconductor packages with annotation and pathway databases to provide comprehensive analysis of RNA sequencing data. Principal component analysis identified one outlier WT sample, which was taken into consideration in subsequent analyses as explained in the publication.