Long-read target region sequencing of the rust resistance gene R11 in sunflower

Sequencing of a 60 kb region spanning the R11 locus on both the R11-HA-R9 and the six R11-susceptible mutants identified three genes corresponding to the genes HanXRQChr13g0422111, HanXRQChr13g0422121, and HanXRQChr13g0422131 annotated in the XRQ reference genome. The first two genes have exactly same sequences in all three genomes, XRQ, R11-HA-R9, and R11-susceptible mutants, while three point mutations were found in the third gene corresponding to HanXRQChr13g0422131 in the three R11-susceptible mutants. First two point mutations caused premature stop codons, and third mutation caused the changes in amino acid from serine to asparagine.