Sequences and bioassay data of the five best peptides from each cycle.

Peptides from the initial population are designated as from cycle 0. In the peptide sequences, wild type amino acids are given in standard font. Exchange by a different L amino acid is represented by a character in bold, D isomers are in addition indicated by lower-case letters. Activities are intracellular calcium mobilization data given as mean of three independent experiments ± standard deviation. Stabilities are peptide half life data from an HPLC assay, given as mean of three independent experiments ± standard deviation. Data in bold indicate the best peptide in this study, showing approximately 70fold higher stability and 2.5fold higher potency than the wild-type peptide chemerin-9.