Structural basis of GAIN domain autoproteolysis and cleavage-resistance in the adhesion G-protein coupled receptors

Structural basis of GAIN domain autoproteolysis and cleavage-resistance in the adhesion G-protein coupled receptors This dataset contains: 1. Molecular Dynamics Dataset serving for the shown MD analysis human ADGRB2 Simulations on the wild-type sequence with histidine in all three hetero states (b2-hsd, b2-hse, b2-hsp), as well as the double mutation S876F/N893G in the same manner (b2-mut-hsd, b2-mut-hse, b2-mut-hsp). rat ADGRL1 uncleaved model MD simulations on the wild-type sequence with histidine in all three hetero states (l1-hsd, l1-hse, l1-hsp) Each trajectory contains the glycosylated protein with a 100 ps timestep (~15000 frames). Further MD data is available upon request. 2. ZIP archives with raw data underlying Figures from the manuscript and supplemental information Figures_6_and_S13.zip Raw scans of the gels used for Figures 6 and S13 Figures_7_and_S15.zip Raw scans of the gels used for Figures 7 and S15 Images of these gels after ImageJ linear scaling Saved files from GelAnalyzer for quantifying the band intensities Excel sheets with compiled data GraphPad Prism analysis of the data Figures_S14.zip Raw scans and GelAnalyzer evaluation of the gels shown in Figure S14 Figures_S16.zip Raw scans of the gels used for Figure S16 sequence_alignments.zip Sequences (fasta format) of the constructs used in the study and multiple squence alignment with annotation for use in program Jalview.