Datasets associated to manuscript "The interplay between Caenorhabditis elegans larval development and Orsay virus infection"

Datasets necessary to reproduce the following Figures: Figure 1. OrV accumulation dynamics. (a) Log10 RNA2 genomes/ng of total RNA measured every 2 h during 24 hpi. The abscissa shows hph. The first molting period (M1) is highlighted by a pink rectangle, and the second molting period (M2) by a lime green one. Blue lines and symbols represent the viral load for nematodes inoculated at 0 hph while green ones represent those inoculated at 6 hph. (b) Same data as in (a) but rescaled according to hpi. Figure 2. Identification of infected cells and viral presence in the intestinal lumen. (b) Percentage of nematodes with smiFISH signal. Nematodes inoculated at 0 hph are shown in blue, and nematodes inoculated at 6 hph are shown in green. Points represent biological replicates (each one corresponding to a plate of animals, each plate containing 40 animals). Mean is represented as a horizontal line, and standard deviation (SD) as a vertical line. (c) Percentage of animals according to the number of infected intestinal cells. Nematodes inoculated at 0 hph are shown in blue, and nematodes inoculated at 6 hph are shown in green. Error bars represent ±95% confidence interval (CI). (d) Percentage of smiFISH signal on cells (in green), lumen (in purple), or both (in yellow), in 0 hph and 6 hph inoculated nematodes. Error bars represent ±95% CI. On this experiment, four replicates were done for each condition, 40 animals per replicate. Figure 3. Impact of viral infection on the expression of development-related genes. (c) Expression profiles of developmental-related genes let-7, lin-42 and nhr-23 along time. Expression profiles of control animals are shown in purple circles and lines, while blue triangles and lines indicate infected animals. Shadowed areas represent ±1 SE. Three replicates per timepoint were done. Figure 4. Impact of viral infection on C. elegans development. (c) Duration of intermolt stages (I1 - I4). (d) Duration of molts (M1 - M4). (e) Time from the last molt (M4) until hatching of progeny. (f) Time from the first intermolt period (I1) until hatching of progeny. In panels (c) - (f) mean value are represented by black horizontal bars. Control nematodes are represented by purple circles while infected ones are represented by blue triangles.