Gene expression profile at single cell level of colonic epithelial cells from the distal colon. Gene expression profile at single cell level of colonic epithelial cells from the distal colon

The colon is densely innervated by nociceptor neurons. Single cell RNA sequencing (scRNA-seq) of colon epithelial cells was performed to analyze the role of nociceptor neurons and Ramp1 signaling in regulating colonic epithelial cells. Overall design: Colonic epithelial cells were purified by Fluorescence-activated cell sorting (FACS) from nociceptor knockout (Nav1.8Cre_P_DTA) mice, epithelial specific Ramp1 knockout (VillinCre_P_Ramp1) mice and their littermate control mice. The purified cells were used for scRNAseq using 10X.