Kalifabougou_Samples_Andrade

We analysed P. falciparum parasites from asymptomatic RDT+ children at the beginning (January), middle (March) and end (May) of the dry season (2018), and of parasites from clinical malaria case in the wet season (MAL) (2018 and 2019), and asymptomatic RDT+ children in the wet season, in October (Oct, 2019). Here, we measured the increase in parasitemia and parasite development at 0, 16, 24, 30, 36 and 48 h after in vitro culture.
Conclusion:
Earlier increase in parasitemia in vitro during the dry season, due to longer circulation of P. falciparum iRBCs in the bloodstream during the dry season. Observation of similar parasite development from asymptomatic children in the dry season and from asymptomatic children in the wet season, when compared to clinical malaria cases in the transmission season.
Notes:
The following samples were mislabelled during sample acquisition. Sample description has correct names January 2018 Specimen_001_24h_437.fcs corresponds to sample k435 24h May 2018 d6230518_30h_k724.fcs corresponds to k721 30h d6230518_36h_k724.fcs corresponds to k721 36h MAL 18 554 ?timepoints from 24h onwards are labelled as k535 from September 13th: 13Sep18_24h_k535 1,3a,50.fcs corresponds to k554 24h with 1 to 50 dilution 13Sep18_30h_k535 1,3a,50.fcs corresponds to k554 30h with 1 to 50 dilution 13Sep18_36h_k535 1,3a,50.fcs corresponds to k554 36h with 1 to 50 dilution 13Sep18_48h_k535 1,3a,50.fcs corresponds to k554 48h with 1 to 50 dilution October 2019 TpointsSilviaoct19_d5 k084 16h_001.fcs corresponds to k084 24h TpointsSilviaoct19_d5 k1053 16h_001.fcs corresponds to k1053 24h TpointsSilviaoct19_d5 k1083 16h_001.fcs corresponds to k1083 24h TpointsSilviaoct19_d5 k271 16h_001.fcs corresponds to k271 24h Both unstained and uninfected controls were used in each Flow cytometer sample acquisition.