Aged gut microbiota lead to gut dysbiosis with a potential to induce obesity in mice. mouse gut metagenome

We have already found that the aging-related alterations in the murine intestinal environment. The features of aged gut microbiota are similar to the intestinal environment of an obese phenotype. To directly ask whether the middle-age gut microbiota contributes to the obese phenotype, we orally transferred fecal microbiota from young or middle-age SPF mice into 8-week-old Germ Free (GF) mice. When placed on a high fat diet, mice gavaged with middle-age SPF fecal microbiota displayed enhanced weight gain and higher blood glucose levels after oral glucose challenge compared to those colonized with young SPF fecal microbiota, although these parameters were comparable on a normal diet. we shows that the profiles of fecal microbiota which mice were transferred fecal microbiota from young or middle-age SPF mice into 8-week-old GF mice.