GAr-dependent inhibition of antigen presentation in different cell lines from different origins.

The presentation of SIINFEKL from chicken ovalbumin (Ova) itself or Ova inserted in the EBNA1 coding sequence was detected using the B3Z reporter cells. The presentation of SIINFEKL from Ova or from an EBNA1 construct that lacks the GAr sequences (EBNA1ΔGA) was given the arbitrary value of 100%. The right column shows the effect of the c-myc IRES on GAr-dependent inhibition of antigen presentation in cell lines from different origins. The table shows data from at least three experiments and SD. The c-myc IRES overrides GAr-dependent antigen presentation in human derived cell lines only. (N.T. = not tested).