IRF8 dependent dendritic cells are essential for intestinal T cell homeostasis. IRF8 dependent dendritic cells are essential for intestinal T cell homeostasis
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB10856
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In the following study the effect of lacking intestinal IRF8-dependent dendritic cells on the gut microbiota composition was investigated. IRF8 was deleted from female C57BL/6 mice using Cre-Lox recombination and compared to IRF8 positive mice. The mice were kept on a low fat diet. When sacrificed cecum and colon samples were collected. DNA was extracted from all samples using Machery-Nagel NucleoSpin Soil kit. Finnally, the microbiota composition was analyzed using 16S rDNA targeted amplicon sequencing on an Illumina Miseq.
创建时间:
2016-07-02



