A novel single TCR gamma chain fused with antibody-based binding domain facilitates T cell cytotoxicity for potent anti-tumor response without forming TCR-CD3 complex

To test the hypothesis that the activation and regulatory signaling in ?-TCR?-T cells could be dissimilar from the conventional TCR-T, we stimulated anti-PD-L1 d-TCR?d- and ?-TCR?-T cells engineered from three different donors, and then compared the genome-wide transcriptome profiles by RNA sequencing (RNA-Seq). Overall design: Comparative gene expression profiling analysis of RNA-seq data for ?-TCR?-T cells (gamma9 group) and d-TCR?d-T cells (g9d2 group) after antigen stimulation