Effects of exemestane on mitochondrial transmembrane potential (ΔΨm) in MCF-7aro cells.

Cells were treated with different concentrations of exemestane with or without 1 mM of 3-MA after 3 and 6 days. Treated cells were harvested and labeled with DiOC6(3) and PI followed by flow cytometry analysis. Data are presented as viable cells and cells with ΔΨm loss. Cells cultured with T at 1 nM were considered as control and cells treated with T plus CCCP (10 µM) were considered as positive control for ΔΨm loss.The data represents means ± SEM of three independent experiments done in triplicate. The ratio treatment/control is presented in bold within brackets. Significant differences between the control versus treated cells, or between the control plus 3-MA versus cells with exemestane plus 3-MA are indicated by***(p