ChEC-seq2: an improved chromatin endogenous cleavage sequencing method and bioinformatic analysis pipeline for mapping in vivo protein–DNA interactions

We validated ChEC-seq2 with MNase-fusions to transcription factors with well-documented motifs and binding sites in S. cerevisiae. ChEC-seq2 with Rap1-MNase, Gcn4-MNase, and Ino2-MNase in S. cerevisiae. Soluble MNase was used as a control. Biological triplicates are provided. *********************************************** Grant for this project: Grant ID: R35 GM136419 Grant Title: The molecular mechanisms and functional significance of gene positioning PI: Jason Brickner