A five-finger exercise: five overlooked non-European species of Tubifera (figures and supplementary files)

Supplementary file S1. Collection and barcoding data for all studied specimens of Tubifera and Siphoptychium. New taxa and GenBank data are shown in bold (xlsx file). Supplementary file S2. Spore measurement of for the studied specimens of Tubifera. A. Spore size of individual specimens. B. Spore size of the species of Tubifera. C. Box and whisker plot showing spore size range in specimens of Tubifera (xlsx file). Supplementary file S3. Concatenated alignment of partial nucSSU (positions 1–1556), mtSSU (1557–2102), and EF1a (2103–2795) sequences (fasta file). Supplementary file S4. Preliminary species delimitation based on ASAP, PTP and recombination analyses. For the PTP and ASAP, each number correspond to the delimited species. For the recombination data, each number for every gene corresponds for the genotype. For ASAP, for each of two genes the five partitions are shown, with the partition score value (line 3) and the number of delimited species (line 4). The partitions with the best (lowest) score are marked in red (xlsx file). Supplementary file S5. Spore size of Tubifera species in different sections of the genus (xlsx file). Fig. 1. Maximum likelihood phylogeny of the Reticulariaceae, based on 120 nucSSU, 48 mtSSU, and 20 EF1a sequences. Branch support is shown as follows: Shimodara-Hasegawa SH-aLRT test / Approximate Bayes test / Ultrafast bootstrap test (1000 replicates). Bold: specimens studied here. Dark star: species described here; light star: species yet undescribed; contoured star: subspecies elevated to species status. Dots: available sequences, black – nucSSU, grey – mtSSU, white – EF1a. Fig. 2. Tubifera annulifera sp. nov. A, B. Pseudoaethalium. C–E. Apices of sporothecae. F. Peridium and spores, stained by methyl blue in lactic acid. G. Outer surface of the peridium, SEM. H. Inner surface of the peridium, SEM. I. Spore, SEM. J. Spores, stained by methyl blue in lactic acid; the last one is shown in optical section. Specimens: A–C, E, G–I. TNSX73283 (Holotype). D. TNSM14811. F. TNSX74103. J. TNSX74103 (1–2), TNSM14811 (3–10). Scale bars: A = 2 mm. B, C = 1 mm. D, E = 0.5 mm. F = 10 µm. G, H = 5 µm. I, J = 1 µm. Photo authorship: D.V. Leontyev. Fig. 3. Tubifera coralloides sp. nov. A. Immature pseudoaethalium. B, C. Pseudoaethalia. D, E. Apices of sporothecae. F. Peridium and spores, stained by methyl blue in lactic acid. G. Outer surface of the peridium, SEM. H. Inner surface of the peridium, SEM. I. Spore, SEM. J. Spores, stained by methyl blue in lactic acid; the last one is shown in optical section. Specimens: A. Not collected. B, D–I. sc34608 (Holotype). B. SJL2350. J. sc34608 (1–2), SJL2350 (3–10). Scale bars: A–C = 1 mm. D = 0.5 mm. E = 0.2 mm. F = 10 µm. G = 5 µm. H–J = 1 µm. Photo authorship: A. S.J. Lloyd. B–J. D.V. Leontyev. Fig. 4. Tubifera digitella sp. nov. A–C. Pseudoaethalia. D, E. Apices of sporothecae. F. Peridium and spores, stained by methyl blue in lactic acid. G. Outer surface of the peridium, SEM. H. Inner surface of the peridium and the spore, SEM. I. Spore, SEM. J. Spores, stained by methyl blue in lactic acid; the last one is shown in optical section. Specimens: A, C, G–I. TNSX73224 (Holotype). B, D–F. TNSX73250. J. TNSX73224 (1–5), TNSX73250 (6–10). Scale bars: A = 2 mm. B, C = 1 mm. D, E = 0.5 mm. F = 10 µm. G = 5 µm. H–J = 1 µm. Photo authorship: D.V. Leontyev. Fig. 5. Tubifera suavis sp. nov. A, B. Pseudoaethalia. C–E. Apices of sporothecae. F. Peridium, stained by methyl blue in lactic acid. G. Outer surface of the peridium, SEM. H. Inner surface of the peridium and the spore, SEM. I. Spore, SEM. J. Spores, stained by methyl blue in lactic acid; the last one is shown in optical section. Specimens: A–C, G–I. sc31869 (Holotype). D. LE325554. E. UARK22187. F. TNSM12824. MM30618 (1–5), sc12444 (6–10). Scale bars: A = 2 mm. B, C = 1 mm. D, E = 0.5 mm. F = 10 µm. G = 5 µm. H–J = 1 µm. Photo authorship: D.V. Leontyev. Fig. 6. Tubifera yamamotoi sp. nov. A, B. Pseudoaethalia. C. Cylindrical (above) and spherical (below) sporothecae. D. Cylindrical sporothecae. E. Spherical sporothecae on the hypothallus of the pseudoaethalium. F. Peridium and spores, stained by methyl blue in lactic acid. G. Outer surface of the peridium, SEM. H. Inner surface of the peridium and the spore, SEM. I. Spore, SEM. J. Spores, stained by methyl blue in lactic acid; the last one is shown in optical section. Specimens: A, C–G, I. TNSX73517. B. TNSX12412. H. CWP2925. J. TNSX73517 (1–2), TNSM12412 (3–10). Scale bars: A, B = 2 mm. C, D = 0.5 mm. E = 0.2 mm. F = 10 µm. G = 5 µm. H–J = 1 µm. Photo authorship: D.V. Leontyev. Fig. 7. Tubifera dimorphotheca. A–D. Pseudoaethalia. E. Apices of cylindrical sporothecae. F. Spherical sporothecae on the hypothallic stalk of the pseudoaethalium. G. Peridium and spores, stained by methyl blue in lactic acid. H. Outer surface of the peridium with a collapsed spore, SEM. I. Inner surface of the peridium, SEM. J. Spore, SEM. K. Spores, stained by methyl blue in lactic acid; the last one is shown in optical section. Specimens: A, E, F, H–J. NENB10675 (Type). B, D. TNSM13565. C. MM9907. D, G. TNSX73253. K. NENB10675 (1–5), TNSX73253 (6–10). Scale bars: A, B = 1 mm. C–E = 0.5 mm. F = 0.2 mm. G = 10 µm. H = 5 µm. I–K = 1 µm. Photo authorship: D.V. Leontyev.