林木根际高效解磷微生物促生抗逆机理与效应研究

本项目针对我国人工林地力衰退现状，首次广泛从我国多省区收集筛选林 木根际有益微生物，开展促生抗逆机理研究，并将其应用于人工林中以调控根圈微生物区系，增强林木健康及抗逆能力，提高人工林生物生产力。主要创新性成果如下： 1.建立了我国首个林木根际解磷细菌与菌根辅助细菌种质资源库。从我国16省区的 杨树松树和枫香等主要栽植区广泛采集土样分离筛选根际有益微生物，共获得杨树解磷细 菌212株、松树解磷细菌248株和枫香解磷细菌50株以及一批菌根辅助细菌，建立了林木根际解磷细菌及菌根辅助细菌种质资源库。 2.确定了林木根际高效解磷细菌的解磷能力，获得一批具有自主知识产权的高效解磷 菌株并完成种类鉴定。通过比较林木根际解磷细菌在不同条件下的解磷能力，筛选获得杨 树松树和枫香根际高效解磷细菌(17种/株)和菌根辅助细菌(14种/株)，并完成种类鉴定。 弄清了各高效解磷菌株的最适解磷条件。 3.探明了高效解磷细菌的解磷特性及其解磷促生的分子机制。阐明了根际高效解磷细 菌解磷能力与产酸性能的关系，解磷细菌与根系互作对有机酸分泌的影响及其酶学特性 发现了一批与植物互作有关的重要基因和解磷相关基因 筛选鉴定出高效解磷细菌新的非编sRNA，该研究国内外未见其他报道。

This project targets the current situation of soil fertility decline in plantation forests in China. For the first time, we extensively collected and screened rhizosphere beneficial microorganisms of forest trees from multiple provinces and regions across China, conducted research on the mechanisms of growth promotion and stress resistance, and applied these microorganisms to plantation forests to regulate the rhizosphere microbial community, enhance forest tree health and stress resistance, and improve the biomass productivity of plantation forests. The main innovative achievements are as follows:
1. Established the first germplasm resource bank of forest tree rhizosphere phosphate-solubilizing bacteria and mycorrhizal helper bacteria in China. Soil samples were extensively collected, isolated and screened from major planting areas of poplar, pine, Liquidambar formosana and other main tree species across 16 provinces and regions in China. A total of 212 strains of poplar phosphate-solubilizing bacteria, 248 strains of pine phosphate-solubilizing bacteria, 50 strains of Liquidambar formosana phosphate-solubilizing bacteria, and a collection of mycorrhizal helper bacteria were obtained, thus establishing the germplasm resource bank of forest tree rhizosphere phosphate-solubilizing bacteria and mycorrhizal helper bacteria.
2. Determined the phosphate-solubilizing capacity of efficient rhizosphere phosphate-solubilizing bacteria for forest trees, obtained a batch of efficient phosphate-solubilizing strains with independent intellectual property rights, and completed their species identification. By comparing the phosphate-solubilizing capacities of forest tree rhizosphere phosphate-solubilizing bacteria under different conditions, efficient phosphate-solubilizing bacterial strains (17 strains/species) and mycorrhizal helper bacterial strains (14 strains/species) from the rhizospheres of poplar, pine and Liquidambar formosana were screened out, and their species identification was completed. The optimal phosphate-solubilizing conditions for each efficient phosphate-solubilizing strain were clarified.
3. Elucidated the phosphate-solubilizing characteristics of efficient rhizosphere phosphate-solubilizing bacteria and the molecular mechanism underlying their phosphate-solubilizing and growth-promoting effects. The relationship between the phosphate-solubilizing capacity and acid-producing performance of rhizosphere efficient phosphate-solubilizing bacteria, the effect of the interaction between phosphate-solubilizing bacteria and plant roots on organic acid secretion, and their enzymatic properties were clarified. A number of important genes related to plant interaction and phosphate-solubilizing processes were discovered, and novel non-coding sRNAs in efficient phosphate-solubilizing bacteria were screened and identified. This study has not been reported elsewhere domestically and internationally.

搜集汇总

背景与挑战

背景概述

该数据集聚焦于林木根际高效解磷微生物的促生抗逆机理与效应研究，旨在应对我国人工林地力衰退问题。它通过从16个省区采集土样，建立了我国首个林木根际解磷细菌和菌根辅助细菌种质资源库，筛选出高效菌株并揭示了其解磷分子机制，包括发现新的非编sRNA。数据集包含2016年的文本数据，公开共享，适用于植物学相关研究。