Gene Expression in the Lrrc10 knockout heart. Mus musculus

LRRC10 is a heart-specific gene required for proper cardiac function. The effects of Lrrc10 deletion on gene expression in the adult mouse heart was investigated. Overall design: Lrrc10 knockout mice or wildtype controls, housed in 12 hour light:12 dark, ad lib feeding and drinking conditions were sacrificed at two months of age for cardiac gene expression analysis. A two color, reference design experiment in which heart RNA from 2 Lrrc10 knockout mice was pooled and labeled with Cy5 and hybridized according to Agilent protocols against a reference pool of RNA madeup from respective tissue taken from 2 month wildtype mice which was labeled with Cy3.