Inducible intestinal epithelial knockout of PRMT1 in mouse model

Many studies investigated the mechanisms that control enteroendocrine cell (EEC) fate determination; however, regulation of EEC development and function are still unclear. Here, We performed RNA-Sequencing analyses on the small intestinal crypts of tamoxifen-inducible intestinal epithelium-specific knockout of PRMT1 and wildtype mice. Our finding for the first time revealed that the epigenetic enzyme PRMT1 controls mouse enteroendocrine cell differentiation, which probably via inhibition of Neurogenin 3-mediated commitment to the EEC lineage. To provide insight into the mechanisms underlying PRMT1 function in the adult intestine, we performed RNA-Sequencing (RNA-Seq) of the small intestinal crypts in tamoxifen-induced intestinal epithelial cell-specific deletion of PRMT1 adult mice and littermates as control at different doses or time point after the first tamoxifen injection.