Extreme mitochondrial reduction in a novel group of free-living metamonads

Metamonads are a diverse group of heterotrophic microbial eukaryotes adapted to living in hypoxic environments. All metamonads but one harbour metabolically altered ‘mitochondrion-related organelles’ (MROs) with reduced functions, however the degree of reduction varies. Here, we generate high-quality draft genomes, transcriptomes, and predicted proteomes for five recently discovered free-living metamonads. Phylogenomic analyses placed these organisms in a group we name the ‘BaSk’ (Barthelonids+Skoliomonads) clade, a deeply branching sister group to the Fornicata, a phylum that includes parasitic and free-living flagellates. Bioinformatic analyses of gene models shows that these organisms are predicted to have extremely reduced MRO proteomes in comparison to other free-living metamonads. Loss of the mitochondrial iron-sulfur cluster assembly system in some organisms in this group appears to be linked to the acquisition in their common ancestral lineage of a SUF-like minimal system Fe/S c..., , , # README: Extreme mitochondrial reduction in a novel group of free-living metamonads

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Genomes, gene models, alignments, HMM profiles and supplementary datasets for the BaSk clade - *Skoliomonas litria*, *Skoliomonas* sp. GEMRC, *Skoliomonas* sp. RCL, and *Barthelona* sp. PCE.

## Description of the data and file structure

**BaSk_clade_genome_genes** folder:

* Genomes (.fsa) and gene models (.fasta and .gff3) for each species of the BaSk clade.

**alignments_hmm** folder:

* **alignments_hmm/phylogenomics -** final phylogenomic alignment along with a .txt file outline the presence/absence of each taxa for each concatenated gene. The .txt file also includes summary statistics for the number of present data (and % data presence) for each taxa and each gene.

-  **alignments_hmm/MRO_protiens** - Alignments (.fas), corresponding HMM profiles, and raw phylogenetic trees relating to searches for MRO proteins, separated into folders according to similar biochemical pathways and functions...