Sequencing of miRXplore Universal Reference (Miltenyi Biotec) RNA using mDOT-seq
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE166931
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In present study we optimized and validated the mDOT-seq approach with miRXplore Universal Reference (Miltenyi Biotec) RNA. The results revealed that the 3' adapters with alkyne moieties attached to the fifth carbon atom of the second cytosine or uracil in ACTC and CUCC sequences, respectively, are best suited for the preparation of RNA sequencing libraries. Using mDOT-seq eight samples with four different 3' alkyne-adapters (two experimental replicates each) were prepared and analysed.
创建时间：
2021-05-20
