Opa1 tg Mouse adipocyte RNA-seq

In mammals, there are three types of adipose tissue, white, brown and beige, that aid inmaintaining global energy homeostasis. White adipose adipocytes store energy, brown adipocytes consume energy by producing heat through uncoupling protein 1 (UCP1)-mediated thermogenesis and beige adipocytes reside within white adipose tissue and upon specific stimuli are fully capable of executing thermogenesis, in a process called browning of white adipose tissue. We examined changes in gene expression following white and browning differentiation of white pre-adipocytes isolated from subcutaneous adipose tissue (SAT) of experimental mice. We used a mouse line in which a moderate, ubiquitous overexpression of the mitochondrial and cristae shaping protein Opa1 was obtained (Opa1tg mice, Cogliati et al., 2013). Pre-adipocytes from SAT of Opa1tg and Wtmice were differentiated to mature adipocytes using medium routinely employed for culturing primary adipocytes, non-supplemented (control cells), or supplemented with the PPAR? agonist rosiglitazone in combination with norepinephrine, whose favor the emergence of beige cells in white pre-adipocyte cultures and increase the expression of UCP1. Cells were harvested after 7 days of differentiation and processed for RNA-sequencing. We also profiled proliferating pre-adipocytes isolated from the SAT of Opa1tg and control mice.