The gut microbiota data of hyperuricemia by influence of fucoidan

Fresh fecal samples were collected, immediately frozen in liquid nitrogen, and stored at -80 °C. Genomic DNA was extracted from the feces of five randomly selected mice from each group. The V3-V4 region of the 16S rRNA gene was amplified, and the resulting products were stored at Majorbio Bio-Pharm Technology Co. Ltd. Sequencing was conducted using the Illumina MiSeq platform, and sequence denoising or operational taxonomic unit (OTU) clustering was performed using the QIIME2 data analysis workflow or Vsearch software. Bioinformatics analysis of the gut microbiota was carried out using the Majorbio Cloud platform. Alpha diversity indices were calculated to assess bacterial richness and evenness. Weighted principal coordinate analysis (PCoA) was employed to visualize differences in intestinal microbial community structures among groups, and on Bray-Curtis dissimilarity was performed to determine statistical significance between groups. Additionally, linear discriminant analysis (LDA) effect size (LEfSe) was used to identify significantly enriched bacterial taxa. PICRUSt2 (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) was used to predict metagenomic function based on OTU representative sequences. KO, pathway, and enzyme (EC) information was obtained from the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database, and the abundance of each functional category was calculated based on OTU abundance.