Research data in support of "A synthetic signalling network imitating the action of immune cells in response to bacterial metabolism"

This repository contains raw data in support of the publication 10.1002/adma.202301562. Please refer to the published paper, available open access, for full information and context. Figure 2 data - Panel b: Confocal image of core-shell particle - Panel d: Absorbance data to probe pH responsiveness of the DNA constructs - Panel c: CD data to probe pH responsiveness of the DNA constructs - Panel g: Representative epifluorescence and bright field images and bright field videos to demonstrate pH-induced particle aggregation Figure 3 data - Panel b: pH and OD data vs E. coli growth time in various glucose concentrations - Panels d, e; Representative epifluorescence images and bright field videos to demonstrate E. coli induced particle aggregation and bacteria trapping - Panel f: OD data vs E. coli growth time Figure 4 data - Panel b: OD data vs E. coli growth time showing effect of netosis-like synthetic pathway - Panel e: Representative bright field and epifluorescence images showing effect of netosis-like synthetic pathway Supplementary Figure 1: Agarose gel demonstrating DNA construct assembly Supplementary Figure 2: DLS data showing pH responsiveness of DNA nanostructures Supplementary Figure 3: UV melting curve data for DNA constructs Supplementary Figure 4: DLS data underpinning pH response curve Supplementary Figure 5: Representative data provided in Figure 2, Panel g folder Supplementary Figure 6: Representative microscopy videos used to generate hydrodynamic radius data with DDM Supplementary Figure 7: OD data vs E. coli growth time at various glucose concentrations Supplementary Figure 8: FITC dextran fluorescence intensity data (used to determine pH) vs E. coli growth time at various glucose concentrations Supplementary Figure 9: Calibration data (FITC dextran fluorescence vs pH) used in Figure 3f and Supplementary Figures 8 and 12 Supplementary Figure 10: FITC dextran fluorescence intensity data (used to determine pH in Figure 3f) vs E. coli growth time at various glucose concentrations Supplementary Figure 11: Representative data provided in Figure 3, Panel d, e folder Supplementary Figure 12: FITC dextran fluorescence intensity data (used to determine pH) vs E. coli growth time Supplementary Figure 13: Calibration data (Fluorescein dT fluorescence vs pH) for fluorescent pH probe linked to DNA particles Supplementary Figure 14: Fluorescent data for pH-induced de-quenching on DNA particles Supplementary Figure 15: OD vs E. coli growth time at various antibiotic concentrations Supplementary Figure 16: Representative data provided in Figure 4, Panel c, folder