Beta vulgaris Transcriptome or Gene expression

Plant Polysaccharide Polyphenol Extraction Kit was used to extract total RNA, and FastKing one-step method was used to remove genomic cDNA first-strand synthesis premix reagent, the cassette performs reverse transcription to synthesize the first-strand cDNA. The PCR products were detected by gel electrophoresis to check the specificity of gene primers. The relative expression levels of genes were detected by the Mx3000P real-time PCR system and the Super Real PreMix Plus kit was used for the qRT-PCR test. Each cDNA sample was performed three technical replicates. Transcriptome sequencing was performed on an illumina Hiseq 4000 platform. Raw data was fiirstly filtered by removing reads contaiiniing adapter and multiiple unknown bases as well as some low-qualiity reads.