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<b>Transcriptomic changes reveal hypoxic stress response in submerged seeds of maize (</b><b><i>Zea</i></b><b> </b><b><i>mays</i></b><b> L.)</b> Supplementary_Data.xlsx

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NIAID Data Ecosystem2026-05-02 收录
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Two inbred lines, B73 and Okcheon Chal-1 (OkC1), and a commercial cultivar, 'Sinhwangok’ were used for this study. Seeds were sterilized with 0.5% sodium hypochlorite for a minute at room temperature and washed with autoclaved distilled water five times. Seeds were imbibed at 20°C for 24 hours and moved to 25°C on petri-dish with wet filter paper or in 50 ml conical tube with half of water for a day which represent air or hypoxic condition, respectively. For imbibition and hypoxia treatment, 25 ml TDW was applied for 10 seeds in 50 ml conical tube. Seeds were sampled at right after finishing imbibition (0D) and 1 days after moving to 25°C petri-dish (A1D) or in water (W1D). Each sampling was triplicated for pooling of five seeds.

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2024-06-30
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