Transcription profiling of rat keratinocytes exposed to a 56Fe ion beam

The purpose of the present work was to examine gene expression patterns in a rat keratinocyte line exposed to a 56Fe ion beam Experiment Overall Design: The cells were exposed to 1.01 geV/nucleon 56Fe ions generated by the NASA Space Radiation Laboratory facility. Data from Affymetrix rat microarrays (RAT 230_2) were processed by BRB ArrayTools 3.3.0 software and the Gene Ontogeny (GO) database was utilized to categorize significantly responding genes.

本研究旨在探究受56Fe离子束照射的大鼠角质形成细胞系中的基因表达模式。实验总体设计如下：细胞暴露于由NASA太空辐射实验室设施产生的1.01 GeV/nucleon 56Fe离子。使用BRB ArrayTools 3.3.0软件处理Affymetrix大鼠微阵列（RAT 230_2）数据，并利用基因本体（GO）数据库对响应显著的基因进行分类。