Single-cell RNA-seq identification of the cellular molecular characteristics of sporadic bilateral clear cell renal cell carcinoma

Bilateral renal cell carcinoma (RCC) is a rare
type of this disease and includes familial bilateral and sporadic bilateral RCCs.
Studying the cellular molecular characteristics of sporadic bilateral is
important to provide a direct guide for clinical treatment. These cellular
molecular characteristics can be expressed at the RNA level, especially at the single-cell RNA
level. Single-cell RNA sequencing (scRNA-seq) was performed on bilateral clear
cell RCC (ccRCC). A total of 3,575 and 3,568
high-quality single-cell transcriptome data were captured from the left and
right tumour tissues, respectively. Gene characteristics were identified by comparing left and right
tumours at the scRNA level. This work presented the complex cellular
environment of bilateral ccRCC by scRNA-seq. Single-cell transcriptome analysis
revealed high similarity in gene expression among most cell types of bilateral RCCs
but significant differences in gene expression among different site tumour
cells. Additionally, the potential biological function of different tumour cell
types was determined by gene ontology (GO) analysis. The transcriptome characteristics of tumour
tissues in different locations at the single-cell transcriptome level were
revealed through the scRNA-seq of bilateral sporadic ccRCC. This work provides new insights into the diagnosis and
treatment of bilateral RCC.