Characterization of day 7 bovine blastocysts of three groups.
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The cell numbers in blastocysts were estimated by counting the total number of nuclei using DAPI, and the number of trophectoderm (TE) nuclei was estimated using immunostaining for CDX2. The cell number of the ICM was assessed as the total number of nuclei minus the number of TE nuclei. The data were shown as Mean ± SEM.a, b, cValues with different superscripts within columns are significantly different from each other (P
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2015-12-02



