Figure 5

Figure 5: Noradrenergic sympathetic nerves innervate the developing kidney. (A) E14.5 wholemount immunostained kidney labeled with tyrosine hydroxylase (TH, magenta) shows that the first innervating axons (TUBB3, green) are TH+. Cytokeratin (yellow) marks the developing collecting duct system. (B) P0 wholemount immunostained kidney labeled with TH (magenta) shows that the TH+ nerves have branched extensively alongside the arterial tree (SMA, green). LRP2 marks the proximal tubules (yellow) and the magnified inset shows TH+ nerves (magenta) navigating around and near proximal tubules (arrowhead). (C) E15.5 kidney section immunostained for TH (magenta) and TUBB3 (green) shows TH+ axons around the vasculature (CD31, yellow; arrowhead). (D) Sections of Pod-Cretg/+;R26tdTomato/tdTomato P0 kidneys show TH+ axons (magenta) near glomeruli (tdTomato, green; arrowhead) and proximal tubules (LRP2, yellow). (E) Image of the adult kidney cortex immunostained for neuropeptide Y (NPY, magenta), TH (green), and the nuclear counterstain DAPI (blue). Glomeruli are encircled in white dotted lines. No TH+/NPY- fibers were observed. Image is representative of n=2 male mice.