2022-02-02_Growth curves 1_ Starved cells plus Cu 50 a 250.tab

Growth curves_ General Method The experiments were done using T. cruzi Dm28c strain. T. cruzi epimastigotes were routinely maintained in mid-log phase by periodic dilutions in Liver Infusion Tryptose (LIT) medium (5 g/L Liver Infusion Broth BD DIFCO™ # 226920, 5 g/L GranuCult® prime Tryptose Broth Millipore #1.03866, 68 mM NaCl, 5.3 mM KCl, 22 mM Na2HPO4, and 0.8 % (w/v) glucose, pH 7.4) supplemented with 10 % FBS (Internegocios S.A. - Buenos Aires, Argentina) and 5 μM hemin (LIT-10 % FBS-5 μM hemin medium), at 28 °C. Cell growth was determined by cell counting in an automatized hemocytometer adapted to count epimastigotes (WL 19 Counter AA, Weiner Lab) and in Neubauer counting chamber. Growth curves - set 1 Epimastigotes were cultured in LIT-10 % FBS-0 μM hemin medium for at least 10 days before the performance of the experiment shown in this set of growth curves. For the experiment, the cells were diluted at a concentration of 5 x 106 parasites/mL and challenged to grow in LIT-10 % FBS-0 μM hemin supplemented with 50-250 μM Cu. Control was performed by adding 5 µM hemin to the same medium (without copper). The number of cells was monitored for 7 days. Copper (Cu) was added as Cu2+ from CuSO4 salt. Authors: Merli, Marcelo Luciano; Cricco, Julia Alejandra.