Next-generation RNA sequencing (RNA-seq) has revolutionized our ability to analyze transcriptomes. Current RNA-seq methods are highly reproducible, but each has biases resulting from different modes o
The high diversity and complexity of the eukaryotic transcriptome makes it difficult to effectively detect specific transcripts of interest. State-of-the-art targeted RNA-seq methods incorporate pre-s
A wide range of sequencing methods have been developed to assess nascent RNA transcription and resolve the single-nucleotide position of RNA polymerase genome-wide. These techniques are often burdened
