Transcriptome after spatial exploration in CA1-specific brain tissue from wildtype and NEIL3-deficient mice

CA1-specific brain tissue from the hippocampal formation was isolated at baseline (homecage condition) and after a spatial exploration paradigm ("open field") in wildtype and NEIL3-deficient mice. NEIL3 is a DNA glycosylase potentially involved in transcription regulation. RNA was extracted and sequenced. Subsequently, these results were correlated to an extracellular live recording of pyramidal cell activity ("place cell recording") in the same genotypes. Wildtype (C57Bl6) and NEIL3-deficient (Neil3-/-, C57Bl6-background) were used. At baseline, 2 wildtype and 2 mutant animals were included. After learning, 3 wildtype and 3 mutant animals were included. In both conditions, animals were exposed to the same housing conditions, food ad libitum and handled by the same experimenters.