Acomprehensivedatasetfor transcriptome, metabolome, and microbiomeof four section poplars under low nitrogen conditions

Here, we presentan integrated dataset which contains root transcriptome, root metabolome, rhizosphere microbiome, and phenotypes from nine representative poplar species of four setions under low nitrogen conditions, which enables precise profiling of the activities of host genes regulating metabolites mediating microbial composition and their interactions with plant phenotypes.We examined eleven representative characteristics encompassing morphological and structural, physiological functional, and component content aspects of all poplar species.Moreover, atotal of 73.7 Gb of root transcriptomicraw data was generated, which, after quality filtering, retained 70.0 Gb of clean data and identified 38,739 expressed genes (TPM > 0). Bacterial community composition across the nine poplar species was investigated for each sample type (bulk soil and rhizosphere soil) using Illumina MiSeq sequencing of the V3–V4region of the 16S rRNA gene. The 54 soil samples identified 30,225 ASVs. We detected and quantified 129 root flavonoids representing diverse chemical classes: 83 flavones and flavonols, 16 flavanones, 7 anthocyanins, 13 isoflavonoids, 4 chalcones and dihydrochalcones, and 6 other flavonoids.