Soils Metagenome. soil metagenome

Shotgun-cloned metagenomic libraries from 16 soils (9 grassland, 7 agricultural) were each expressed in E. coli. Those DNA fragments conferring tolerance against inhibitors associated with lignocellulose pretreatment were isolated, sequenced, assembled, and analyzed. Each soil library was selected against a panel of 15 lignocellulosic inhibitors and 5 biofuels.