Single-cell RNA profiling of mammary gland tumours in MMTV-PyMT mouse model of breast cancer.

Here, we use single cell sequencing to delineate the heterogeneity of tumours from the genetically engineered MMTV-PyMT mouse model of breast cancer (MMTV-PyMT::K14Cre::Rosa-tdTomato mice). Mammary tumours were collected from 13-14-week-old MMTV-PyMT female mice and were subjected to single cell expression profiling to address heterogeneity in metastatic breast cancer.