High-throughput sequencing of LPS-stimulated mouse macrophage Raw264.7 pretreated by Lespedeza bicolor root components

In this study, mouse macrophage Raw264.7 was pretreated with Lespedeza bicolor root components and then stimulated with LPS and then high-throughput sequencing technology was used to explore the expression of inflammation-related genes of Raw264.7 in mouse macrophages treated with Lespedeza bicolor root components, and to find interesting targets,NF-kappaB and p38 signaling pathway. RAW 264.7 cells were seeded in 100 mm dishes at a density of 1 × 107 cells per well.Three experimental groups, Control, LPS, and LB+ LPS, were set up, and 0.5 μg/mL LPS was added to stimulate for 6 h after LB was activated for 30 min. After treatment, the cells were collected, and total RNA from each group was extracted using TRIzol (TaKaRa, Japan). Paired-end sequencing was ultimately carried out on an Illumina HiSeq 4000 platform at LC-BIO Technologies (Hangzhou, China).