miRNA profile regulated by SOX2 in glioma stem-like cell

Patient-derived glioma stem-like cell (GSC-11), a kind gift of Dr. Lang at UT MD Anderson Cancer Center, was subjected to a transient transfection to down-regulate SOX2 expression. Specific human SOX2 siRNA and a non-targeting control siRNA (si-Scramble) were used in four independent experiments. The cells were then cultured for 72 h after transfection and subjected to the miRNA array analysis. miRNA array profile of 2 conditions and 1 replicate per condition. Patient derived glioma stem-like cell were used and treated as indicated in the summary: GSC11 cells were transfected with a siRNA against SOX2 or a non-targeting control siRNA (si-Scramble). Equal amount of total RNA from each sample was pooled prior to miRNA expression analysis.