The Polycomb Group protein Rnf2/Ring1b is essential for zebrafish development and cardiogenesis

Goal of the experiment was to assess the differences in gene expression between zygotic rnf2 mutant zebrafish embryos and wildtype embryos at 3 dpf. The goal of ChIP-sequencing of wildtype embryos at 3 dpf is to link deregulation in gene expression to the Rnf2 occupancy in the wildtype situation and check the overlap between Rnf2 and H3K27me3. The RNA-sequencing of single hearts was performed to assess differences in gene expression between zygotic rn2 mutants hearts and wildtype hearts at 3 different stages (1, 2, 3 dpf) RNAseq of 3dpf Danio rerio whole embyos, wild types and rnf2 mutants (8 and 7 replicates, respectively); ChIPseq for rnf2 in 3dpf Danio rerio wild type (2 replicates) and rnf2 mutant (1 sample) embryos (all 3 with Drosophila spike in); ChIPseq for H327me3 in 3dpf Danio rerio wild type (2 replicates) and rnf2 mutant (2 replicates) embryos; ChIPseq for H3K27me3 in 3dpf Danio rerio wild type (2 replicates) and rnf2 mutant (1 sample) embryos (all 3 with Drosophila spike in); RNAseq of 1, 2 and 3 dpf Danio rerio dissected hearts, wild types (9, 13 and 10 replicates, respectively) and rnf2 mutants (8, 9 and 9 replicates, respectively)