Mapping snoRNA-target RNA interactions in an RNA binding protein-dependent manner with chimeric eCLIP [RNA-seq]

To quantify the effect of SNORD89 on splicing in human cells, we compared polyA mRNA-seq results in SNORD89 KD cells and control cells. Overall design: We knock down SNORD89 with antisense oligonucleotide and perfrom polyA mRNA-seq data in none treated cells and SNORD89 KD cells. Alternative splicing was then quantified under two conditions.