Additional file 1: of Identification and co-evolution pattern of stem cell regulator miR394s and their targets among diverse plant species

Figure S1. Statistical analysis using Kolmogorov-Smirnov test through GeneDoc v2.7.0. Calculation of percent identity of aligned miR394 sequences for statistical significance. A. The test has shown that the ≥0.3 fraction of mature miR394 sequences have ≥90% sequence identity B. ~ 0.2 fraction of the precursor pre-MIR394 sequences have > 55% sequence identity. Figure S2. An unrooted ML phylogeny of miR394s along with reverse complementary sequences of miR394–3p using MEGA6. This tree contains reverse complementary sequences of miR394–3p (highlighted, encircled portion). All miRNA394/miR394 − 5 ps are highly conserved with very less substitution rate. The scale bar represents the nucleotide substitution rate. (ZIP 377 kb)