Assembly statistics and BLAST results against nr for the G. bimaculatus de novo transcriptome assembly.

1Values for number of raw reads, number and % of raw reads assembled (passed quality filters described in main text), number of contigs, isotig N50, % of singletons, total number of assembly products, and number of unique BLAST hits against nr, are shown in Table 1.2Because isogroups are collections of isotigs that are hypothesized to originate from the same gene, they do not comprise a single sequence and so cannot be mapped to nr using BLAST.3nr = NCBI non-redundant database.4For BLAST against nr the E-value cutoff was 1e-5. For breakdown of BLAST hits among different classes of assembly sequences, see Table 3.